Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid
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Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid. / Kirstahler, Philipp; Bjerrum, Søren Solborg; Friis-Møller, Alice; la Cour, Morten; Aarestrup, Frank M; Westh, Henrik; Pamp, Sünje Johanna.
I: Scientific Reports, Bind 8, 4126, 2018.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid
AU - Kirstahler, Philipp
AU - Bjerrum, Søren Solborg
AU - Friis-Møller, Alice
AU - la Cour, Morten
AU - Aarestrup, Frank M
AU - Westh, Henrik
AU - Pamp, Sünje Johanna
PY - 2018
Y1 - 2018
N2 - Advances in genomics have the potential to revolutionize clinical diagnostics. Here, we examine the microbiome of vitreous (intraocular body fluid) from patients who developed endophthalmitis following cataract surgery or intravitreal injection. Endophthalmitis is an inflammation of the intraocular cavity and can lead to a permanent loss of vision. As controls, we included vitreous from endophthalmitis-negative patients, balanced salt solution used during vitrectomy and DNA extraction blanks. We compared two DNA isolation procedures and found that an ultraclean production of reagents appeared to reduce background DNA in these low microbial biomass samples. We created a curated microbial genome database (>5700 genomes) and designed a metagenomics workflow with filtering steps to reduce DNA sequences originating from: (i) human hosts, (ii) ambiguousness/contaminants in public microbial reference genomes and (iii) the environment. Our metagenomic read classification revealed in nearly all cases the same microorganism that was determined in cultivation- and mass spectrometry-based analyses. For some patients, we identified the sequence type of the microorganism and antibiotic resistance genes through analyses of whole genome sequence (WGS) assemblies of isolates and metagenomic assemblies. Together, we conclude that genomics-based analyses of human ocular body fluid specimens can provide actionable information relevant to infectious disease management.
AB - Advances in genomics have the potential to revolutionize clinical diagnostics. Here, we examine the microbiome of vitreous (intraocular body fluid) from patients who developed endophthalmitis following cataract surgery or intravitreal injection. Endophthalmitis is an inflammation of the intraocular cavity and can lead to a permanent loss of vision. As controls, we included vitreous from endophthalmitis-negative patients, balanced salt solution used during vitrectomy and DNA extraction blanks. We compared two DNA isolation procedures and found that an ultraclean production of reagents appeared to reduce background DNA in these low microbial biomass samples. We created a curated microbial genome database (>5700 genomes) and designed a metagenomics workflow with filtering steps to reduce DNA sequences originating from: (i) human hosts, (ii) ambiguousness/contaminants in public microbial reference genomes and (iii) the environment. Our metagenomic read classification revealed in nearly all cases the same microorganism that was determined in cultivation- and mass spectrometry-based analyses. For some patients, we identified the sequence type of the microorganism and antibiotic resistance genes through analyses of whole genome sequence (WGS) assemblies of isolates and metagenomic assemblies. Together, we conclude that genomics-based analyses of human ocular body fluid specimens can provide actionable information relevant to infectious disease management.
U2 - 10.1038/s41598-018-22416-4
DO - 10.1038/s41598-018-22416-4
M3 - Journal article
C2 - 29515160
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 4126
ER -
ID: 213166663